Jour : 2 juin 2025

Implementing External Quality Control in PCR Workflows: A Step-by-Step Laboratory Guide

Polymerase Chain Reaction (PCR) has revolutionized molecular biology and microbial detection. Yet the high sensitivity of PCR also makes it vulnerable to technical errors, false positives, and reagent degradation. To ensure accurate, reproducible, and high-confidence results, External Quality Control (EQC) must be embedded into every PCR workflow. This in-depth guide explains how laboratories can implement

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Digital PCR and the Evolution of Positive Controls in High-Sensitivity Applications

Digital PCR (dPCR) is redefining nucleic acid quantification by delivering absolute quantification, unmatched sensitivity, and robust partitioned reactions. This powerful tool is essential in rare mutation detection, viral load monitoring, and copy number variation studies. Central to its success is the use of positive controls—carefully calibrated materials that ensure precision and validity in each dPCR

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