Integratie van microfluïdische monstervoorbereiding met PCR-detectie om de effecten
Klinisch nut van Droplet Digital PCR om BCR-ABL1-transcripten van patiënten met Philadelphia-chromosoom-positieve aigu lymfoblastische leukemie te monitoren Put up-chimere antigeenreceptor 19/22 T-celcocktailtherapie
La leucémie aiguë lymphoblastique à chromosome Philadelphie ( LAL Ph + ) représente 20 à 30 % des sufferers adultes atteints de LAL, caractérisée par une translocation de t (9, 22) . Les inhibiteurs de la tyrosine kinase (ITK) ont considérablement amélioré les résultats, même s’il existe encore des problèmes, notamment des rechutes dues à des mutations résistantes aux médicaments et à une profondeur de rémission moléculaire sous-optimale. Auparavant, nous avons signalé l’innocuité et l’efficacité de la perfusion séquentielle de l’ immunothérapie des cellules T du récepteur de l’antigène chimérique CD19/22 (CAR-T) dans le traitement des néoplasmes à cellules B en rechute/réfractaires (R/R), y compris les cas avec Ph + TOUT. Compte tenu de la possibilité d’une réaction plus profonde, on s’attendait à ce que davantage de sufferers atteignent une réponse optimale de maladie résiduelle minimale (MRD). Une méthode different, la PCR numérique à gouttelettes duplex (ddPCR) à haute sensibilité a été établie, qui pourrait fournir une quantification absolue de la MRD sans avoir besoin de courbes d’étalonnage. Ici, nous avons collecté rétrospectivement 95 échantillons de moelle osseuse de 10 sufferers atteints de R/R Ph + , qui ont reçu 19/22 CAR-T-cell thérapie cocktail.
Notamment, la rémission moléculaire séquentielle pendant plus de three mois (SMR3), un indicateur significatif basé sur la ddPCR après la perfusion de CAR-T a été établie, qui a été définie comme une rémission moléculaire séquentielle pendant pas moins de three mois avec une MRD négative. Dans cette cohorte, aucune récidive n’a été observée chez six sufferers ayant obtenu une SMR3, dont quatre ont accepté une allogreffe de cellules souches hématopoïétiques (allo-HSCT) après un régime de cellules CAR-T. Malheureusement, les quatre autres sufferers qui n’ont pas atteint le SMR3 ont rechuté et n’ont pas reçu de traitement spécifique supplémentaire à l’ exception du régime CAR-T.. En résumé, la ddPCR peut être une different, surtout lorsque l’acide nucléique était insuffisant en pratique clinique. L’absence de SMR3 peut être un avertissement précoce d’une rechute potentielle après CAR-T et indiquer l’initiation d’autres thérapies, y compris l’allo-HSCT.
Kwantitatieve detectie et monitoring van Colletotrichum siamense in rubberbomen rencontré behulp van PCR en temps réel
Colletotrichum siamense est l’un des brokers pathogènes les plus importants de l’hévéa en Asie. La détection et la quantification adéquates des populations de C. siamense dans les hévéas sont importantes pour le suivi des épidémies de la maladie. Dans cette étude, nous avons développé une méthode de PCR en temps réel basée sur l’ITS pour détecter efficacement l’ hévéa infectant C. siamense , qui a détecté de manière fiable aussi peu que 100 fg d’ADN génomique, 100 copies d’ADN cible et 20 conidies. Le protocole PCR en temps réel a reconnu tous les isolats de C. siamense collectés dans trois provinces de Chine , tandis qu’aucune amplification n’a été observée avec l’hévéa et ses autres brokers pathogènes. Détection et quantification de C. siamense ont été réalisées sur des feuilles de caoutchouc infectées artificiellement et naturellement.
Nous pouvions encore détecter C. siamense dans des mélanges de plantes dont seulement 0,0001 % des tissus étaient infectés. Une accumulation d’ ADN de C. siamense a été observée pendant tout le processus d’an infection aux trois stades phénologiques des feuilles, suggérant que la méthode PCR en temps réel peut être utilisée pour surveiller le développement de C. siamense dans les arbres à caoutchouc. Enfin, la méthode a permis la détection de C. siamense dans des feuilles d’hévéas naturellement infectées et asymptomatiques dans les champs. Par rapport aux méthodes de détection antérieures, la méthode PCR en temps réel est plus spécifique et plus wise, et sera d’une grande utilité pour les études visant à mieux comprendre l’épidémiologie de la maladie foliaire à Colletotrichum, ainsi que la prédiction du risque de maladie et la proposition de contrôle.
Integratie van microfluïdische monstervoorbereiding met PCR-detectie om de effecten van gelijktijdige scheiding van DNA-remmers en uitwisseling van DNA-oplossingen te onderzoeken
Dans cet article, nous avons appliqué un dispositif microfluidique à canal incurvé pour séparer l’ADN de l’eau contenant des inhibiteurs de PCR et les laver simultanément dans de l’eau propre pour la détection à l’aide d’un thermocycleur PCR transportable. L’ échantillonnage de l’ADN environnemental (eDNA) est devenu une approche d’enquête efficace pour détecter les organismes rares. Cependant, les molécules d’ADNe à faible focus peuvent être masquées par des inhibiteurs de PCR lors de l’amplification et de la détection, ce qui augmente le risque de fake négatifs. Par conséquent, des applied sciences pour la séparation et le lavage de l’ADN sur website sont nécessaires de toute urgence. Notre dispositif consistait en un microcanal en demi-cercle avec une entrée d’échantillon d’inhibiteur d’ADN , une entrée de tampon propre et plusieurs sorties.
En utilisant les forces d’inertie induites par le flux, des microparticules conjuguées à l’ADN de 10 m ont été concentrées sur la paroi interne du microcanal incurvé tandis que la séparation des microparticules conjuguées à l’inhibiteur de 1 m et le lavage de l’ADN ont été réalisés simultanément avec le flux de Dean. Nous avons réalisé une focalisation, une isolation et un lavage singleplex de particules de 10 μm avec une efficacité de 94,5 ± 2,0 % . Dans des expériences en duplex avec des particules de 1 m et 10 m, des particules plus grosses ont été lavées avec une efficacité de 92,1 ± 1,6 % et une pureté de 79 ± 2 %.
En fonctionnalisant en floor les microparticules avec des groupes d’affinité contre l’ADN de saumon atlantique et l’acide humique (HA), et en traitant des échantillons de différentes concentrations dans notre appareil, nous avons réalisé une purification et une détection efficaces des molécules d’ADN à l’aide du thermocycleur PCR transportable. Notre méthode a significativement diminué les cycles de quantification PCR de Cq > 38 à Cq = 30,35 ± 0,5, ce qui a confirmé l’amélioration de l’amplification PCR. Le dispositif proposé fait un pas en avant prometteur dans la préparation d’échantillons vers un dispositif intégré qui peut être utilisé pour la purification et l’échange simultanés de resolution d’ADN dans des purposes de surveillance environnementale au level de besoin.
Description: A polyclonal antibody against GPR119. Recognizes GPR119 from Human. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/10000
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-GPR119 . This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody for detection of GPR119 from Human. This GPR119 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human GPR119 at AA rangle: 160-240
Description: A polyclonal antibody for detection of GPR119 from Human. This GPR119 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human GPR119 at AA rangle: 160-240
Description: A polyclonal antibody for detection of GPR119 from Human. This GPR119 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human GPR119 at AA rangle: 160-240
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR119 (aa186-235). This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR119 (Cytoplasmic Domain). This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR119 (Cytoplasmic Domain). This antibody is tested and proven to work in the following applications:
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined.
Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer.
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012]
Description: This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene.
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.
ELISA kit for Human Anti-AsAb (Anti-Anti-Sperm Antibody Antibody)
Description: A competitive Inhibition ELISA kit for detection of Anti-Anti-Sperm Antibody Antibody from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
SARS-CoV-2-serologie verhoogt diagnostische nauwkeurigheid bij CT-vermoede, PCR-negatieve COVID-19-patiënten tijdens pandemie
Contexte : En l’absence de détection par PCR de l’ ARN du SRAS-CoV-2 , un diagnostic précis de COVID-19 est difficile. La tomodensitométrie (TDM) à faible dose détecte les infiltrats pulmonaires avec une sensibilité élevée, mais les résultats peuvent être non spécifiques. Cette étude évalue la valeur diagnostique de la sérologie SARS-CoV-2 pour les sufferers présentant des caractéristiques CT distinctes mais une PCR négative.
Méthodes : Un immunodosage chimioluminescent IgM/IgG a été réalisé chez 107 sufferers atteints de COVID-19 confirmé (groupe A : PCR+ ; CT ±) et 46 sufferers suspectés (groupe B : PCR- répétitives ; CT+) de COVID-19, admis dans un hôpital universitaire allemand. lors de la première obscure de la pandémie. Une classification CT standardisée et interne des signes radiologiques d’une pneumonie virale a été utilisée pour évaluer la probabilité de COVID-19 .
Résultats : Les taux de séroconversion (SR) déterminés aux jours 5, 10, 15, 20 et 25 après l’apparition des symptômes (SO) étaient de 8 %, 25 %, 65 %, 76 % et 91 % pour le groupe A et 0 %, 10 % , 19 %, 37 % et 46 % pour le groupe B, respectivement ; (p < 0,01). Par rapport aux sufferers hospitalisés avec une évolution non compliquée (sufferers non-USI), la séroconversion avait tendance à se produire à une fréquence plus faible et retardée chez les sufferers dans les unités de soins intensifs. Le SR des sufferers avec des résultats CT classés comme haute certitude pour COVID-19 était de 8 %, 22 %, 68 %, 79 % et 93 % dans le groupe A, contre 0 %, 15 %, 28 %, 50 % et 50 % dans groupe B (p < 0,01). La sérologie SARS-CoV-2 a établi un diagnostic définitif chez 12/46 sufferers du groupe B. Chez 88 % (8/9) des sufferers avec une sérologie négative > 14 jours après l’ apparition des symptômes (groupe B), la réévaluation de consensus clinico-radiologique a révélé des diagnostics probables autres que COVID-19 . La sensibilité de la sérologie SARS-CoV-2 était supérieure à la PCR > 17 jours après l’apparition des symptômes.
Conclusions : Environ un tiers des sufferers présentant des résultats distincts de la tomodensitométrie COVID-19 sont testés négatifs pour l’ARN du SRAS-CoV-2 par PCR, ce qui rend difficile un diagnostic right. La mise en œuvre des checks sérologiques SARS-CoV-2 aux côtés des algorithmes de diagnostic actuels basés sur la CT/PCR améliore la discrimination entre les infiltrats pulmonaires liés au COVID-19 et non liés chez les sufferers négatifs à la PCR. Cependant, la sensibilité de la sérologie SARS-CoV-2 dépend fortement du second du check et devient supérieure à la PCR après la 2 e semaine suivant l’apparition des symptômes.
Description: A polyclonal antibody against GPR110. Recognizes GPR110 from Human. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/40000
Description: A polyclonal antibody for detection of GPR110 from Human. This GPR110 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human GPR110 at AA range: 810-890
Description: A polyclonal antibody for detection of GPR110 from Human. This GPR110 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human GPR110 at AA range: 810-890
Description: A polyclonal antibody for detection of GPR110 from Human. This GPR110 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human GPR110 at AA range: 810-890
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR110 . This antibody is tested and proven to work in the following applications:
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined.
Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer.
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012]
Description: This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene.
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.
ELISA kit for Human Anti-AsAb (Anti-Anti-Sperm Antibody Antibody)
Description: A competitive Inhibition ELISA kit for detection of Anti-Anti-Sperm Antibody Antibody from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
